Double-Muscled Phenotype in Mutant Sheep Directed by the CRISPRCas9 System
نویسندگان
چکیده
منابع مشابه
Targeted mutations in myostatin by zinc-finger nucleases result in double-muscled phenotype in Meishan pigs
Myostatin (MSTN) is a dominant inhibitor of skeletal muscle development and growth. Mutations in MSTN gene can lead to muscle hypertrophy or double-muscled (DM) phenotype in cattle, sheep, dog and human. However, there has not been reported significant muscle phenotypes in pigs in association with MSTN mutations. Pigs are an important source of meat production, as well as serve as a preferred a...
متن کاملCompensatory Growth in Double Muscled Bulls
COMPENSATORY GROWTH IN DOUBLE MUSCLED BULLS 1 2 3 Different Periods of Feed Restriction Before Compensatory Growth in Belgian Blue Bulls: 4 II. Plasma Metabolites and Hormones 5 6 J. L. Hornick, C. Van Eenaeme, M. Diez, V. Minet, and L. Istasse. 7 8 9 10 Department of Nutrition, Veterinary Faculty, Sart Tilman 11 B43 4000 Liège, Belgium 12 13 Phone: 32-(0)4-3664139 14 Fax: 32-(0)4-3664122 15 E-...
متن کاملMolecular expression of myostatin and MyoD is greater in double-muscled than normal-muscled cattle fetuses.
Excessive muscling in double-muscled cattle arises from mutations in the myostatin gene, but the role of myostatin in normal muscle development is unclear. The aim of this study was to measure the temporal relationship of myostatin and myogenic regulatory factors during muscle development in normal (NM)- and double-muscled (DM) cattle to determine the timing and possible targets of myostatin ac...
متن کاملthe effect of e-64 on the developmental competence of sheep cocs during in vitro maturation
in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.
Characterization of gene expression in double-muscled and normal-muscled bovine embryos.
Myostatin, a member of the transforming growth factor-beta superfamily, is a negative regulator of skeletal muscle growth. Cattle with mutations that inactivate myostatin exhibit a remarkable increase in mass of skeletal muscle called double muscling that is accompanied by an equally remarkable decrease in carcass fat. Although a mouse knockout model has been created which results in mice with ...
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ژورنال
عنوان ژورنال: Cloning & Transgenesis
سال: 2018
ISSN: 2168-9849
DOI: 10.4172/2168-9849.1000161